Cultivation of Human Chondrocytes on Cartilage Fluff Matrix without Loss of Phenotypic Expression
نویسندگان
چکیده
منابع مشابه
The ECM-Cell Interaction of Cartilage Extracellular Matrix on Chondrocytes
Cartilage extracellular matrix (ECM) is composed primarily of the network type II collagen (COLII) and an interlocking mesh of fibrous proteins and proteoglycans (PGs), hyaluronic acid (HA), and chondroitin sulfate (CS). Articular cartilage ECM plays a crucial role in regulating chondrocyte metabolism and functions, such as organized cytoskeleton through integrin-mediated signaling via cell-mat...
متن کاملCD147 (Extracellular Matrix Metalloproteinase Inducer-EMMPRIN) Expression by Human Articular Chondrocytes
Background: Integrins are a family of transmembrane proteins that allow communication between the extracellular matrix and the interior of cells. Chondrocytes, cells of articular cartilage, express integrins and these molecules appear to have a variety of roles including mechanotransduction. Integrins are known to associate with a number of accessory molecules such as CD147 that may act to regu...
متن کاملMultipotent Stromal Cells Outperform Chondrocytes on Cartilage-Derived Matrix Scaffolds
OBJECTIVE Although extracellular matrix (ECM)-derived scaffolds have been extensively studied and applied in a number of clinical applications, the use of ECM as a biomaterial for (osteo)chondral regeneration is less extensively explored. This study aimed at evaluating the chondrogenic potential of cells seeded on cartilage-derived matrix (CDM) scaffolds in vitro. DESIGN Scaffolds were genera...
متن کاملExpression of cartilage-specific molecules is retained on long-term culture of human articular chondrocytes.
Normal human adult articular chondrocytes were used to determine how the chondrocyte phenotype is modulated by culture conditions following long-term culture. We report here for the first time that human articular chondrocytes have a lifespan in the range of 34-37 population doublings. While chondrocytes cultured as monolayers displayed a fibroblastoid morphology and grew faster, those cultured...
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We describe the isolation and the ultrastructural characteristics of adult bovine articular chondrocytes in vitro. Slices of bovine articular cartilage undergo sequential digestions with pronase and collagenase in order to release cells. Chondrocytes are plated at high density (1 x 105 cells/cm 2) in culture dishes or roller bottles with Ham's F-12 medium, supplemented with 10% fetal bovine ser...
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ژورنال
عنوان ژورنال: Journal of Transplantation Technologies & Research
سال: 2014
ISSN: 2161-0991
DOI: 10.4172/2161-0991.1000138